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Ideally, epitope vaccines really should consist of B cell epitopes that encourage a protecting antibody response as well as important T cell epitopes that stimulate cytotoxic T lymphocyte (CTL) generation along with a Th immune response. The ultimate sequence of our vaccine applicant was an antigenic, nonallergenic protein which was soluble when overexpressed in E. coli
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was designed by choosing 9 domains full of B and T cell epitopes and linking them towards the EAAAKEAAAK linker. The sequence selected like a vaccine candidate creates a protein composition that is certainly stable and effective at interacting Along with the TLR2 and TLR4 receptors from the immune system as an immunogen and elicits the right probable reaction to deliver helpful defense.
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